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KMID : 1024420140180040332
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Food Engineering Progress
2014 Volume.18 No. 4 p.332 ~ p.340
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Bioconversion of Puffed Red Ginseng Extract Using β-Glucosidase-producing Lactic Acid Bacteria
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Sim Gun-Sub
Park Gwi-Gun
Park Young-Seo
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Abstract
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Lactic acid bacteria producing high levels of β-glucosidase were screened to convert the major ginsenosides into theminor ginsenosides in puffed red ginseng. A strain named YLB 8 isolated from doenjang showed the highest β-glucosidase activity and was identified as Leuconostoc mesenteroides. The optimum conditions for the maximumproduction of β-glucosidase, when L. mesenteroides YLB 8 was cultured in MRS medium, were as follows; glucoseconcentration of 1% (w/v), initial pH 8.0, culture temperature of 30oC, culture time of 16 h. When Puffed red gin-seng extract was bioconverted using L. mesenteroides YLB 8, the optimum temperature and the concentration ofPuffed red ginseng extract was 30oC and 12oBx, respectively. As a result of bioconversion, the major ginsenosidesRb1 and Rb2 were converted into minor ginsenoside Rg3, and Re was converted into Rg3 and successively intoRh1 and Rh2 in part.
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KEYWORD
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puffed red ginseng extract, bioconversion, β-glucosidase, lactic acid bacteria
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